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Fig. 5. Effects of overexpression of wild-type centaurin ${alpha}$ 1 in developing dissociated hippocampal neurons. Hippocampal neurons were transfected by Nucleofection after dissociation (0 DIV) and cultured for 3, 7 and 14 DIV. (A) Fluorescence (GFP, green) and indirect immunofluorescence ( $beta$ -tubulin; red, 3 DIV; blue, 7 and 14 DIV; Flag-tagged centuarin ${alpha}$ 1, red) were used to visualize transfected neurons and neuronal morphology. GFP control (left panels). GFP plus Flag-centaurin ${alpha}$ 1 (right panels). Insets show higher magnification. Bars, 20 µm. (B) Quantification of the effects of expression of FLAG-centaurin ${alpha}$ 1 at 3 and 7 DIV compared with GFP control. The data was quantified from three independent experiments (n=50 cells from 3 DIV and n=30 cells from 7 DIV, respectively, for each condition). Data represent mean ± s.e. ^*P<0.05.

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