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Figure 5


Fig. 5. The NGF-induced activation of N-WASP is mainly dependent on Cdc42. (A) Irregularity indexes (average ± s.e.m.) of NGF-induced PC12 cells transfected with control or Cdc42 siRNA (left). The almost complete siRNA-mediated knockdown of Cdc42 for Cdc42 relative to actin (right) is shown in the western blot. (B,C) Representative images of N-WASP biosensor expressing PC12 cells co-transfected with Cdc42 siRNA (B) or co-expressing constitutively active HA-tagged Cdc42-G12V (C). Intensities of CFP (Donor), YFP (Acceptor) and the FRET ratio are shown (left) for representative cells; cumulative FRET ratios are shown for both conditions as in Fig. 4 (right). Bars, 5 µm (untreated cells), 10 µm (NGF-treated cells).





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