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Figure 1


Fig. 1. Expression of DDB2-EYFP and EGFP-CUL4A. (A) Localization of DDB2 and CUL4A. The upper panel shows the distribution of DDB2-EYFP in an MRC5 cell, the middle image shows the distribution of EGFP-CUL4A in a HeLa cell and the lower images show the localization of endogenous DDB2 and CUL4A detected with specific antibodies in primary human cells (VH10). (B) Representation of the DNA constructs encoding fluorescently tagged DDB2 and CUL4A. (C) Western blot analysis of MRC5-SV40 cells expressing DDB2-EYFP. Whole cell extracts (10 µg) of non-UV-irradiated cells and UV-irradiated cells (2 and 4 hours after irradiation with 20 J/m2), were probed with antibodies against DDB2, DDB1 and CUL4A.





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