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Figure 2


Fig. 2. FRAP analysis of DDB2 mobility in non-damaged cells (NoDa). (A) Example of a DDB2-EYFP cell in which a strip (2 µm) spanning the nucleus was bleached. (B) Recovery plots of DDB2-EYFP (n=14) and XPG-EGFP (n=23), normalized between 0 and post-bleach intensity after full equilibration. (C) Normalized recovery plots of DDB2-EYFP at 37°C and 27°C (n=12), DDB2(R273H)-EYFP (n=13) at 37°C and EGFP-CUL4A (n=11) at 37°C. (D) Recovery of DDB2-EYFP normalized to pre-bleach intensity (red line). Monte Carlo simulation assuming a diffusion constant of 2.4 µm2/second (green line) and the residuals (blue lines at the bottom) that are a measure for the quality of the simulation. (E) Recovery of XPG-EGFP normalized to pre-beach intensity (red line), Monte Carlo simulation assuming a diffusion constant of 4.7 µm2/second (green line) and the residuals (blue lines).





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