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Figure 5


Fig. 5. UV-C laser irradiation. (A) GFP-XPA expressing cells were irradiated with 266 nm either without (arrow) or with attenuation (arrowhead). GFP-XPA accumulates on both areas (green, left panel) whereas TUNEL (red, middle panel) only stains positive on the spot that was created without attenuation. (B) GFP-XPA expressing cells were irradiated by attenuated UV-C laser light (arrow). Presence of CPDs was shown by immunohistochemical staining with {alpha}-CPD (red, middle panel). (C) Cells that were irradiated in G1 or G2 phase (homogeneous PCNA pattern, red, middle panel) show no accumulation of Rad54-GFP (green, left panel) 2 hours after irradiation (arrow). In cells that were irradiated in S phase (PCNA pattern in foci, red, middle panel) Rad54-GFP (green, left panel) accumulates at locally irradiated areas within 1 hour after irradiation (arrow).





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