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Figure 1


Fig. 1. dab-1 loss-of-function mutations. (A) Schematic representation of the dab-1 gene showing the two isoforms predicted by expressed sequence tag sequences; one of these isoforms is spliced to the SL1 trans-spliced leader RNA. The region deleted by the gk291 mutation is indicated. Exons are represented by numbered boxes, and splicing patterns by diagonal lines. (B) Schematic of the predicted longer DAB-1 isoform (the two isoforms differ only in their extreme N-termini) showing the regions affected by the two dab-1 mutations. The phosphotyrosine binding domain is indicated by the grey box, and the positions of putative adaptor protein and Eps15 homology (EH) domain binding sites are shown: DPF and NPF, respectively. The horizontal line indicates the extent of the isoform-specific region of the N-terminus. (C) Western blot of wild-type (N2), dab-1(gk291)- and dab-1(hu186)-derived lysates showing absence of wild-type DAB-1 immunoreactivity in mutant worm lysates. The antibody recognises several prominent, non-specific bands in both wild-type and mutant worms, one of which is visible here (asterisk). The position of the 50 kDa marker is indicated.





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