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Figure 3


Fig. 3. Localisation of DAB-1 and RME-2 in developing oocytes. Deconvolved image stacks showing detection of DAB-1 and RME-2 in dissected oocytes. DAB-1 and RME-2 localise to distinct structures associated with the plasma membrane of wild-type oocytes (A-F; A-C, upper focal plane; D-E, middle focal plane). Inset in C shows magnified view of the merged images. dab-1(gk291) oocytes (G-H, upper focal plane; I-J, middle focal plane) lack RME-2 structures seen in wild-type oocytes, although diffuse, membrane-associated RME-2 can still be detected (H,J). Note the lack of immunoreactivity with DAB-1 antibody (G,I). Bar, 10 µm.





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