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Figure 3


Fig. 3. Recruitment of MLN51 to SGs does not require its association with the exon junction complex. (A) Schematic representation of MLN51-derived constructs, indicating different consensus motifs including the SELOR module. The white and grey boxes show the position of the N- and C-terminal constructs, respectively. (B) Immunofluorescence analysis of HeLa cells transfected with full length and truncated EYFP-tagged MLN51 proteins (green). Arsenite-treated cells were fixed 24 hours, after transfection. (C) Immunofluorescence analysis of HeLa cells transfected with EYFP-tagged MAGOH (a,c, green) and Y14 (d,f, green). Arsenite-treated cells were stained with an anti-FMRP antibody (b,e, red). Nuclei were counterstained with Hoechst (blue).





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