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Figure 5


Fig. 5. Kinetochore proteins in anoxia. Syncytial blastoderm embryos were incubated in either normoxic (+O2) or anoxic (–O2) buffer for 20 minutes before fixation and immunolabeling. (A) Anti-Cenp-C (Cenp-C) and DNA stain (DNA). (B) EGFP-Nuf2 (Nuf2) and DNA stain (DNA) in gEGFP-Nuf2 embryos. In response to anoxia, Cenp-C and EGFP-Nuf2 accumulate on spindles predominantly in the centrosome-proximal region. Bar, 10 µm.





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