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Figure 7


Fig. 7. Mps1 modification in response to anoxia. gEGFP-Bub3 embryos were fixed and labeled with DNA stain (DNA) after a 20-minute incubation in either normoxic (+O2) or anoxic (–O2) buffer. Syncytial blastoderm embryos during interphase (i) or metaphase (m) were sorted using a microscope. Anoxic interphase embryos were further sorted into early interphase (ie) and late interphase (il) based on the appearance of chromatin that is condensed into a meshwork during early interphase and into distinct chromatids during late interphase. Sorted embryos were used for extract preparation and immunoblotting with anti-Mps1 (Mps1) and anti-GFP (Bub3).





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