JCS009506 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 -

  Adobe PDF

• Movie 1 -

  Movie 1. Normal mitosis. Spindle in rsw7 after 7 hours at the restrictive temperature, undergoing mitosis similar to that of the wild type. The movie shows metaphase, anaphase and telophase, and spans 11 minutes in total.

• Movie 2 -

  Movie 2. Collapse of a bipolar spindle. Spindle in rsw7 after 7 hours at the restrictive temperature, undergoing collapse at anaphase after starting out bipolar. The bipolar spindle appears looser and less focussed than a wild-type spindle through prometaphase before the poles collapse together, forming a radial spindle. After spindle collapse, the DNA appears as a dark mass in the centre of the cell and microtubules, seemingly originating from the collapsed spindle, move towards the cell periphery in an apparent attempt to form a phragmoplast. The movie spans 17 minutes in total.

• Movie 3 -

  Movie 3. Extremely abnormal spindle. Spindle in rsw7 after 28 hours at the restrictive temperature. The spindle is very broad and unstable, and dispersed throughout the cell. Small bipolar mini-spindles form intermittently within the array, which ultimately collapses into a monopole, and the microtubules disperse to the edges of the cell. The movie spans 26 minutes in total.