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Figure 4


Fig. 4. Confocal micrographs of fixed cells with immunolabeled spindles. (A-F) Spindles double labeled for microtubules (green) and DNA (red). (A) Wild-type cells. Typical bipolar spindles in (clockwise from the upper left) anaphase, metaphase and telophase. (B) rsw7 cells grown at 19°C. Most spindles resembled those of the wild type, but a few were aberrant, such as the multi-polar spindle at the top of the panel. (C-F) rsw7 cells exposed to 30°C for 16-24 hours illustrating the range of morphologies, including radial (C,E,F) and linear (D). Radial spindles varied from compact (F) to diffuse (C) and chromosomes were seen either at the centre or periphery of the radial spindle. (G-L) Double labeling for {alpha}-tubulin (green) and {gamma}-tubulin (red). In the wild type (G-I), {gamma}-tubulin is concentrated at the poles at prophase (G) and anaphase (I), but dispersed though the spindle at metaphase (H). In rsw7 (J-L), {gamma}-tubulin is focused at the poles at prophase (J), spread throughout the diffuse spindles (K), and at the centre of compact, radial spindles (L). Bars, 5 µm.





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