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Fig. 5. Pre-prophase bands and phragmoplasts in rsw7 cells. Confocal micrographs of preprophase bands and phragmoplasts in cells of rsw7 plants exposed to the restrictive temperature for 24 hours (A-D) and 6 hours (E,F) prior to fixation. (A,B) Preprophase bands. (C) Cell with an enlarged nucleus and incomplete cross wall. (D) Cell with curved and asymmetrically placed, but structurally normal, phragmoplast. The cell margin is marked with a dashed line. (E) Cell with an aborted cell wall (arrowhead) and unusually deployed phragmoplast fragments. (F) Cell with central DNA mass and radially deployed phragmoplast fragments, possibly reflecting a stage following a spindle as shown in Fig. 4L. Such residual microtubule structures sometimes appeared to be associated with fragments of cell plate (arrowhead). Bars, A,B 5 µm; C-F 10 µm.