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Figure 6


Fig. 6. Mitosis in live rsw7 cells. Single frames from an image sequence of GFP-tubulin in the rsw7 background. For the complete sequence see Movie 2 in supplementary material. The seedling had been exposed to 30°C for approximately 7 hours by time zero (min:sec, in upper left). This spindle was more or less bipolar to start with, although the poles were less focused than normal. The spindle appeared to be in a prometaphase-like state (0 to 3:00) before the poles rapidly collapsed towards each other (6:00 to 10:30). After a pause in the monopolar configuration, the microtubules migrated away from the poles towards the edges of the cell (12:00 to 13:30), leaving the chromosomes at the centre (visible as a dark mass). Bar, 5 µm.





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