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Figure 2


Fig. 2. ZRP-1 appears at the leading edges of migrating cells. (A) Xenopus A6 cells constitutively expressing EGFP-ZRP-1 (left) were fixed and stained with anti-vinculin antibody (middle). A merged image is shown on the right. (B) Behavior of ZRP-1 in migrating living cells. A6 cells constitutively expressing EGFP-ZRP-1 were grown to confluence and wounded. After 6 hours, cells were imaged at 5-minute intervals. The arrowheads indicate the appearance of EGFP-ZRP-1 at the tip of the leading edge. Bars, 15 µm.





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