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Figure 4


Fig. 4. ZRP-1-depleted cells exhibit aberrant actin dynamics. (B) HeLa cells were treated with control siRNA (upper panel) or ZRP-1 siRNA (lower panel) for 24 hours. Cells were then transfected with EGFP-actin expression vector. Forty-eight hours after vector transfection, cells were imaged at 1-minute intervals (A). Bars, 15 µm. (B) Percentages of cells lacking actin stress fibers. (C) Among the cells lacking actin stress fibers, the emergence rates of cells displaying aberrant actin polymerization were measured. n indicates the number of cells examined.





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