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Figure 6


Fig. 6. Shroom2 targeting requires the presence of TJs. (A) In non-confluent MDCK cells, GFP-mShrm2 displays a punctate labeling, that perfectly colocalizes with F-actin (red) in the cytoplasm or beneath the plasma membrane. (B) In nascent cell-cell contacts, as shown in the peripheral cells of MDCK islands, GFP-mShrm2 is recruited to the plasma membrane at the AJC. GFP-mShrm2 is present in some (arrowheads) but not all junctional regions visualized by ZO-1 labeling (arrows). (C) Upon cell confluency, GFP-mShrm2 spreads to all junctional sites, as shown in Caco-2 cells. In these cells, GFP-tagged shroom2 colocalizes with occludin and ZO-1 but not with beta-catenin; see confocal orthogonal (YZ) reconstructions at the bottom.





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