Reduced migration, altered matrix and enhanced TGF1 signaling are signatures of mouse keratinocytes lacking Sdc1

JCS03480 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S 1. Total integrin expression is similar in wt and sdc-1 null keratinocytes. Integrin steady-state levels in wt and sdc-1-null keratinocytes show no differences in the overall amount of eight different integrins when normalized for equal amount of of total protein (in μg) and for actin expression.

• Supplemental Figure S2 -

  Fig. S2. sdc-1-null keratinocytes show evidence for elevated constitutive upregulation of TGFβ1-responsive mRNAs. Semiquantitative RT-PCR shows that the sdc-1-null keratinocytes express higher levels of mRNAs for TGFβ1, and for several TGFβ1-inducible mRNAs encoding proteoglycans and ECM proteins compared with wt keratinocytes. Data were normalized to a control mRNA in each independent assay. To plot the data from each experiment, data were normalized, and values for wt keratinocytes adjusted to a value of 1 to emphasize the fold increase in RNA for the sdc-1-null keratinocytes. These data show a representative experiment. However, Table 1 shows the combined data from a minimum of three different experiments on RNA isolated from different sets of cells. Differences that are significant for the mRNAs indicated by asterisks in Table 1, are also indicated by asterisks here. CN, collagen; FMN, fibromodulin; FN, fibronectin; TN-C, tenacin-C; TSP, thrombospondin.