QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 5. Disruption and activation of TGF1 signaling has distinct effects on the migration rates of wt and Sdc1-null keratinocytes. (A) wt and Sdc1-null keratinocytes were grown for 3 days, after which keratinocytes were treated with a TGF1 neutralizing antibody overnight and then tracked by time-lapse microscopy the next day. Data show that neutralizing TGF1 reduced wt keratinocyte migration rates by over 50% but inhibited Sdc1-null keratinocyte migration by less than 30% compared with untreated or control IgG treated Sdc1-null keratinocytes. (B) wt and Sdc1-null keratinocytes were grown for 3 days, after which keratinocytes were treated with 0.25 or 2.5 ng/ml TGF1 overnight and then tracked the next day. The migration rates of the Sdc1-null keratinocytes were restored to those of wt keratinocytes after treatment with 0.25 ng/ml TGF1. Further, the Sdc1-null keratinocytes migrated significantly faster than the wt keratinocytes when given 2.5 ng/ml TGF1. Whereas lower concentration of TGF1 stimulated wt keratinocyte migration rates, higher concentration did not. ^*P<0.05; grey line highlights values above untreated wt controls. (C) Localization of LN-332 and 4 integrin in migrating wt and Sdc1-null keratinocytes 24 hours after treatment of keratinocytes with either TGF1-neutralizing antibody (a-d) or with 0.25 ng/ml of TGF1 (e-f). Bar, 5 µm.

Return to article