|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 6. Sdc1-null keratinocytes have increased expression of several different integrins on their surface but, unlike wt keratinocytes, they do not increase their integrin surface expression in response to TGF
1. (A) Flow cytometry analysis on unfixed isolated wt and Sdc1-null keratinocytes revealed increased surface expression of 1 and 4 integrins, as determined using Student's t-test (P< 0.05). (B) Tests of biotinylation efficiency show that the amount of biotin incorporated per ng total protein for wt and Sdc1-null keratinocytes is similar, as is the overall profile of biotinylated proteins. (C,D) Biochemical analyses of surface integrins using biotinylation and immunoprecipitation reveals elevated levels of several integrins in untreated Sdc1-null keratinocytes, excluding 
3 integrin; the increase seen for 2 integrin was not significant. Treating wt and Sdc1-null keratinocytes with 0.25 ng/ml TGF1 for 24 hours significantly increased the expression in wt keratinocytes of all the integrins tested, excluding 31, but had no significant effect on integrin surface expression by the Sdc1-null keratinocytes. Numbers in C represent fold increase in surface integrins in the untreated and TGF1-treated Sdc1-null keratinocytes relative to wt keratinocytes.
|
