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Figure 2


Fig. 2. Microscopic demonstration of the presence of SNAREs and CGRP in rat TGNs. Cells were grown on coverslips for 7 DIV, fixed and permeabilised prior to labelling with specific primary and secondary antibodies (as described for Fig. 1). Confocal images after labelling with antibodies against SNAP25 (1:500), CGRP (1:500), syntaxin I (1:500) and Sbr II (1:1000) demonstrated punctate staining of these proteins and the neuropeptide in cell bodies and/or their processes. Merged images are shown below each pair of singly stained micrographs.





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