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Figure 6


Fig. 6. BoNT/D blocks evoked Ca2+-dependent CGRP release and cleaves three Sbr isoforms. The amount of Ca2+-dependent basal and evoked release of CGRP for each stimulus was measured in cells treated with BoNT/D, as described for Fig. 4. (A) Three Sbr isoforms were detected using antibodies against Sbr I or Sbr II, or Sbr I, Srb II and Srb III together (HV-62); Srb I and Srb II co-migrate. (B) Dose-response curves for the remaining intact Sbr II ({blacktriangledown}) and Sbr I ({Delta}), and for inhibition of release evoked by 60 mM K+ ({blacksquare}); inset shows the amount of CGRP in TGNs after overnight incubation with or without BoNT/D. (C) Dose-response curves for BoNT/D-induced inhibition of CGRP release evoked by 1 µM capsaicin (bullet) or 0.1 µM bradykinin ({blacktriangleup}). Inset shows the reduction of basal release by BoNT/D. The values are the mean ± s.e.m.; n=8.





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