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Fig. 3. Adipogenic induction of dermal fibroblasts. (A) Dermal fibroblasts were cultured in adipogenic-inducing (left panel) medium or in regular culture medium (right panel) for 3 weeks. Oil Red O staining is positive in the adipogenic-induced cells. (B) RT-PCR shows PPAR-
2 and leptin mRNA expression in cells with adipogenic induction for 3 weeks. No expression was found in non-induced cells. Bars, 100 µm.