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Fig. 6. Differentiation potential of dermal fibroblast clones. Single-cell-derived clones were expanded for over 25 doublings and then induced in differentiation media for different time intervals as described in the Materials and Methods. Adipogenesis was evaluated by Oil Red O staining. Osteogenesis was revealed by alkaline phosphatase staining (ALP) and Alizarin Red staining (ARS). Chondrogenesis was revealed by Safranin O staining and immunostaining of type II collagen (Col II). Forty-seven clones were analyzed and numbers and percentages of different lineage types are listed. A/O/C, adipogenic/osteogenic/chondrogenic potential.