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Figure 8


Fig. 8. One multipotent dermal fibroblast clone exhibits neurogenic and hepatogenic differentiation potentials. (A) After 10 days of neurogenic induction, cells change to a neuron-like shape and express neurofilament-M (NF-M), neurotensin receptor 3 (NTR3) and neuron-specific class III-tubulin (betaIII-tubulin) in Clone 96A14, but not in Clone 96A33 or Clone 96A29 (data not shown). (B) After 3 weeks of hepatogenic differentiation, cells express albumin (ALB), CK18 and hepatocyte nuclear factor-3 beta (HNF-3beta) in Clone 96A14. No hepatocyte markers are positive in Clone 96A33, even though a slight morphological change is observed. Neither morphological change nor hepatocyte markers are observed in Clone 96A29 (data not shown). Bars, 100 µm.





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