spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Ultrastructural analysis. Nuclear formation was performed as before with cytosol and light membranes. Reactions were run with mock-treated membranes along with either buffer (control) or in the presence of 100 µM {alpha}-SNAP. Shown are results for {alpha}-SNAPL294A that were identical to {alpha}-SNAPwt (data not shown). In parallel, a reaction was performed with membranes pretreated with NEM, but without exogenous {alpha}-SNAP (NEM). Samples were fixed, processed and imaged by transmission electron microscopy. Note the flattened cisternae and nuclear pores (arrowheads) in control reactions and the tight attachment of vesicles (arrows) upon inhibition with {alpha}-SNAP, but not with NEM. Bars, 500 nm.





Right arrow Return to article