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Figure 7


Fig. 7. RanQ69L addition cannot overcome the arrest of NPC assembly caused by inhibition of membrane fusion. Sperm chromatin was incubated with the 200,000 g supernatant of egg extract. Reactions were performed after addition of buffer, 5 µM RanQ69L, RanQ69L plus 100 µM {alpha}-SNAPL294A or RanQ69L plus 0.1% Triton X-100, as indicated. Samples were fixed and chromatin, membranes and FxFG-containing nucleoporins were visualized with DAPI, DiOC6 or by fluorescently labeled mAb414 antibody, respectively. Note the increased recruitment of membrane and FxFG-nucleoporin in the presence of RanQ69L, but not with RanQ69L in combination with detergent or excess {alpha}-SNAP.





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