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Figure 6


Fig. 6. Diagram of noa-lacZ reporter constructs. Grey lines represent flanking sequences of the noa gene. Their extent is indicated by the start nucleotide of the sequence. Grey boxes represent transcribed regions of the noa gene (for orientation pA signals are indicated in the 3' UTR). The lacZ coding region is shown in black. The top two constructs contain only a portion of the 5' UTR and a synthetic ATG in front of the lacZ coding region. Termination of transcription occurs in the hsp70 3' UTR (white box). The constructs in the middle contain the entire 5' UTR with the noa translation start codon together with varying sizes of the promoter region. The constructs at the bottom contain varying lengths of the noa 3' UTR. The relative intensities of resulting beta-galactosidase activity are indicated on the right and refer to expression in all tissues.





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