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Fig. 8. Noa expression in cyst cells is dependent of germ cells. Expression patterns are compared for postmeiotic cyst cell expression in noa^l(3)01895/+ testes (A,C,E,G) and premeiotic expression in l(3)04708/+ testes (B,D,F,H).The normal enhancer trap pattern is shown in adult (A,B) and in larval testes (C,D). Apical expression is indicated by a double arrow in A,C and E. The larval testis anlagen demonstrate the postmeiotic expression in noa^l(3)01895/+, since they contain only premeiotic stages of spermatogenesis and show no staining in cyst cells. In addition, expression in the nuclei of the fat body cells is visible (C). Premeiotic expression, starting with the progenitor cells, is clearly visible in larval l(3)04708/+ testes (D). The enhancer trap pattern is altered but -galactosidase activity can still be observed in bgcn mutant testes (E,F) and in germ cell free tudor testes (G,H). The patterns are distinctly different and in the case of l(3)04708/+ correlate well with premeiotic expression (F). The modification in the pattern is due to the altered testis shape resulting from lack of elongated stages. In the case of noa, expression at the testis base is apparently due to activity in the cells of the terminal epithelium (E,G), as can best be seen in G, where the various parts of the testis base are well extended. The beginning of the terminal epithelium is indicated by an arrowhead, the beginning of the seminal vesicle by an arrow in A,E,G,H. Note that enhancer trap activity is in the terminal epithelium in noa^l(3)01895/+ testes, but clearly above that region in l(3)04708/+ testes (G,H).

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