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Figure 1


Fig. 1. Ceramide activates GSK-3beta. 10I cells were treated with 25 µM of C2-ceramide for 1, 2, 4 and 6 hours with or without 10 mM LiCl. We used western blotting to determine GSK-3beta phosphorylation (P-GSK-3beta) at serine 9 and glycogen synthase phosphorylation (P-GS) at serine 641. Total GSK-3beta and GS proteins were the control. The relative ratio of P-GSK-3beta:total GSK-3beta and of hyperphosphorylated GS (hyper P-GS):total GS are shown. beta-actin levels were determined as an internal control.





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