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First published online 31 July 2007
doi: 10.1242/jcs.016253
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Research Article |
1 Wellcome Trust Centre for Stem Cell Research, Tennis Court Road, Cambridge, CB2 1QR, UK
2 Cambridge Antibody Technology, Milstein Building, Granta Park, Cambridge, CB1 6GH, UK
3 CR-UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge, CB2 0RE, UK
* Author for correspondence (e-mail: fiona.watt{at}cancer.org.uk)
Accepted 12 June 2007
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Summary |
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 Top Summary IntroductionResultsDiscussionMaterials and MethodsReferences |
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Key words: Delta, Cell-cell adhesion, Epidermis, Stem cell, Syntenin
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Introduction |
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TopSummaryIntroductionResultsDiscussionMaterials and MethodsReferences |
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; Watt et al., 2006). Distinct populations of stem cells reside in the hair follicle, sebaceous gland and interfollicular epidermis (Owens and Watt, 2003; Watt et al., 2006).
Notch signalling plays an essential role in cell fate determination during embryogenesis and also in postnatal life (Artavanis-Tsakonas et al., 1999; Baron, 2003). Following binding of ligand (Delta or Jagged), Notch undergoes cleavage of its intracellular domain. The intracellular domain translocates to the nucleus, where it binds to Suppressor of Hairless Su(H) (RBP-J/CBF1 in vertebrates) to activate transcription (Baron, 2003).
In mammalian epidermis, Notch signalling regulates differentiation and has a tumour suppressor function (Lefort and Dotto, 2004). Activation of Notch in cultured interfollicular epidermal keratinocytes can stimulate terminal differentiation (Lowell et al., 2000; Rangarajan et al., 2001). Notch signalling is required for postnatal hair follicle development (Blanpain et al., 2006; Estrach et al., 2006; Pan et al., 2004; Vauclair et al., 2005) and inappropriate activation results in perturbation of tissue homeostasis (Lin et al., 2000; Uyttendaele et al., 2004). Epidermis lacking Notch1 shows increased susceptibility to developing chemically induced tumours (Nicolas et al., 2003).
In the epidermis, three Notch ligands – Delta1, Jagged1 and Jagged2 – display temporal and spatial regulation of expression (Favier et al., 2000; Powell et al., 1998). Delta1 is expressed in human interfollicular epidermis and cultured human keratinocytes (Lowell et al., 2000). In human interfollicular epidermis there is evidence for clustering of stem cells in the basal layer, and Delta1 expression is highest in the putative stem cell clusters (Jensen and Watt, 2006; Jensen et al., 1999; Lowell et al., 2000).
By overexpressing Delta1 in cultured human keratinocytes we have identified two distinct activities of Delta1 (Lowell et al., 2000; Lowell and Watt, 2001). High Delta1 expression by undifferentiated putative stem cells signals to neighbouring cells to differentiate into transit-amplifying cells. High Delta1 expression also promotes keratinocyte cohesiveness, defined as the tendency of groups of cells to remain in contact with one another; this effect requires the cytoplasmic domain of Delta1 (Lowell et al., 2000; Lowell and Watt, 2001). Although many of the events downstream of Notch activation in the epidermis have been characterised (Mammucari et al., 2005; Pan et al., 2004), little is known about the mechanism by which Delta1 promotes epidermal cell cohesiveness. This is an important issue, because the location of stem cells in particular sites within the epidermis determines the local microenvironmental signals they receive, which direct stem cells to self-renew or differentiate along distinct lineages (Fuchs, 2007; Owens and Watt, 2003; Watt et al., 2006). In the present experiments, we have established that the cohesive effects of Delta1 in keratinocytes are mediated via the PDZ-binding domain and involve the adaptor protein syntenin. We also reveal an unexpected role of syntenin in Notch activation.
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Results |
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). We compared full-length DeltaD (DeltaFl) with a mutant that lacks all but 13 of the amino acids of the cytoplasmic domain (DeltaDS; equivalent to mouse DeltaT) (Henrique et al., 1997) (Fig. 1A). Delta1 has a conserved motif, ATEV*, at its C-terminus, which fits the consensus for a PDZ-domain-binding protein (Wright et al., 2004). To test the functions of this sequence, we generated a deletion (DeltaLC) and a point mutation of the PDZ-binding domain (DeltaVA) (Fig. 1A). The point mutation has been previously described to disrupt the interaction between a PDZ-binding domain and its partner (Lin et al., 1999).
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Following binding of a ligand (Delta or Serrate/Jagged), Notch undergoes cleavage of its intracellular domain and translocates to the nucleus, where it binds to RBP-J to activate transcription of HES genes (Baron, 2003). To test the ability of the Delta mutants to activate Notch signalling, we measured luciferase activity driven by the HES1 promoter (Jarriault et al., 1995). J2-3T3 cells stably transduced with the different Delta constructs were transiently transfected with the luciferase reporter and 48 hours later luciferase activity was measured (Fig. 1C). Western blotting of cell lysates established that the different Delta constructs were expressed at similar levels (Fig. 1C).
DeltaFl and DeltaDS activated the HES promoter to the same extent, whereas DeltaVA had much stronger activity (Fig. 1C). The activation of Notch observed in this experimental setting will result from the combined effects of activation in trans (i.e. between neighbouring cells) and inhibition in cis (in the same cell) (Artavanis-Tsakonas et al., 1999; Glittenberg et al., 2006). Thus, the activation of Notch induced by Delta VA could reflect an increase in the activation in trans and/or reduced inhibition in cis.
Colony-forming efficiency (CFE) and percentage of abortive clone formation can be used as indicators of epidermal stem cell activity in culture. Stem cells establish actively growing clones in which self-renewal and differentiation take place, whereas abortive clones are attributable to transit-amplifying cells that undergo terminal differentiation within a few rounds of division (Lowell et al., 2000). Keratinocytes were transduced with the Delta constructs and plated on wild-type (WT) feeders (Fig. 1D; Table 1). DeltaFl and DeltaDS had no effect on CFE, consistent with our previous studies (Lowell et al., 2000), although the size of clones formed by keratinocytes expressing DeltaFl was increased (Fig. 1D). By contrast, keratinocytes expressing DeltaVA showed a strong reduction in CFE and an increase in the proportion of abortive clones (Fig. 1D; Table 1). Thus, the enhanced Notch activation by DeltaVA (Fig. 1C) correlated with an inhibition of clonal growth, consistent with the ability of Notch to promote epidermal differentiation (Blanpain et al., 2006; Rangarajan et al., 2001; Vauclair et al., 2005).
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Boundaries between populations of cells with high and low Notch signalling activity are known to be important in Drosophila (Artavanis-Tsakonas et al., 1999; Glittenberg et al., 2006). As previously reported, culturing WT human keratinocytes on feeder cells (J2-3T3) transduced with Delta1 led to a reduction in CFE and an increase in the proportion of abortive clones (Lowell et al., 2000) (Table 2; see also schematic summary in Fig. 6). Expression of DeltaFl and DeltaDS in J2-3T3 had the same effects (Fig. 1E; Table 2; Fig. 6). Replacement of DeltaFl-expressing feeders with WT feeders after 5 days did not restore keratinocyte colony formation, showing that the effect was irreversible, consistent with stimulation of terminal differentiation (data not shown). WT keratinocytes cultured on DeltaVA feeders showed a small reduction in CFE and increase in abortive clones compared with cells cultured on WT feeders; nevertheless, the effects were much less dramatic than the effects of culture on DeltaFl feeders (Fig. 1E; Table 2; Fig. 6).
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These results establish that a point mutation in the Delta1 PDZ-binding domain has different effects on keratinocyte differentiation to full-length Delta1 or deletion of the entire cytoplasmic domain. DeltaFl and DeltaDS can only promote differentiation in trans (Delta-expressing feeders, WT keratinocytes), whereas DeltaVA triggers differentiation in cis (Delta-expressing keratinocytes) (Fig. 6).
Although WT keratinocytes and cells transduced with DeltaFl or DeltaDS had similar clonal growth properties, they differed in their programme of terminal differentiation in an in vitro reconstituted human skin model (Fig. 1F-H). Primary human keratinocytes transduced with empty vector, DeltaFl and DeltaDS were cultured for 16 days at the air-liquid interface on a human dermal substrate (DED) (Janes et al., 2004). Keratinocytes transduced with empty vector reconstituted an epidermis comprising basal, spinous, granular and cornified layers, thereby resembling normal epidermis (Fig. 1F). Keratinocytes expressing DeltaFl also formed a multilayered epidermis, but it lacked granular and cornified layers (Fig. 1G). By contrast, the epidermis formed by keratinocytes transduced with DeltaDS had a reduced number of spinous layers, a prominent granular layer and an increased number of cornified layers (Fig. 1H). Consistent with the histological appearance, transduced epidermis of WT, Delta FL and Delta DS had normal expression of 
6 integrin, a basal layer marker (Fig. 2D-F). DeltaFl promoted proliferation (Fig. 2A-C) and expression of spinous layer markers (keratin 10, Fig. 1I-K; transglutaminase 1, Fig. 2G-I), whereas DeltaDS promoted expression of granular markers (loricrin; Fig. 2J-L). The effects of DeltaDS are in good agreement with the finding that a soluble form of the Notch ligand Jagged1 stimulates loricrin expression and cornified envelope formation (Nickoloff et al., 2002).
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) (Fig. 3A). Expression of DeltaFl promoted cohesiveness (Fig. 3A,B), whereas DeltaDS promoted scattering of clones of cells, consistent with the effects of mouse Delta1 and DeltaT (Fig. 3A,B) (Lowell et al., 2000). Deletion (DeltaLC) or mutation (DeltaVA) of the PDZ-binding domain also led to reduced cohesiveness (Fig. 3A,B). We conclude that the ability of Delta to promote keratinocyte cohesiveness is mediated by the PDZ-binding domain and that cohesiveness is regulated independently of Notch activation (compare Fig. 1C with Fig. 3B).
To investigate the mechanism by which Delta1 mediates keratinocyte cohesiveness, a two-hybrid screen was performed using the cytoplasmic domain of Delta1, including the PDZ-binding domain, as bait. Several putative partners were isolated, the most highly represented being syntenin (Table 3). Syntenin is a cytoplasmic scaffold protein, containing two PDZ domains. It interacts with the actin cytoskeleton and with cell adhesion receptors, including syndecan and integrins (Gao et al., 2000; Grootjans et al., 1997; Oh and Couchman, 2004; Zimmermann et al., 2001). Syntenin is reported to colocalise with E-cadherin and syndecan-1 at epithelial cell-cell contacts (Zimmermann et al., 2001).
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To validate the association between syntenin and Delta1, a syntenin-GST fusion protein was incubated with lysates of A431 cells that had been transfected with the different Delta constructs (Fig. 3C). Only DeltaFl bound syntenin in the pull-down assay, DeltaDS, LC and VA did not, thereby establishing a requirement for the Delta PDZ domain (Fig. 3C). The interaction between syntenin and Delta1 was also observed in pull-down assays involving GST-syntenin and endogenous Delta1 protein in A431 cells (Fig. 3D; note that the upper band in the lysate lane is non-specific; compare middle lane of Fig. 3E).
To establish that endogenous syntenin bound endogenous Delta1, lysates of A431 cells were subjected to immunoprecipitation with anti-syntenin antibody or, as a negative control, anti-laminin 5 antibody, and then western blotted with an antibody to the Delta1 extracellular domain (Fig. 3E). Endogenous Delta1 was co-immunoprecipitated with endogenous syntenin. In some lysates, anti-Delta1 antibody specifically recognised two prominent bands, with only the upper band being associated with syntenin (Fig. 3E). It is likely that the lower band is the cleaved extracellular domain of Delta1. These results confirmed the direct interaction between syntenin and Delta1 and showed that the interaction is mediated by the C-terminal PDZ-binding domain of Delta1.
Syntenin is reported to be one of the genes that are highly upregulated in the reservoir of stem cells in the mouse hair follicle bulge (Tumbar et al., 2004). We therefore examined whether syntenin was upregulated in the clusters of stem cells in human interfollicular epidermis previously shown to express high levels of Delta1 (Jensen and Watt, 2006; Lowell et al., 2000). Syntenin localised to cell-cell borders in cultured keratinocytes (Fig. 3F) and in the basal layer of human interfollicular epidermis (Fig. 3G). Within the epidermis, syntenin expression was more abundant in the clusters of basal cells that lie closest to the tissue surface, which is the location of the putative IFE stem cells (Fig. 3G). Thus in both human and mouse epidermis there is evidence for upregulation of syntenin in stem cell populations.
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We hypothesised that if the reduction in clonal growth observed with DeltaVA (Fig. 1D) is indeed due to enhanced Notch signalling, then keratinocytes transduced with DeltaFl and Rnai1 should also show reduced clonal growth. Rnai1 did not affect the CFE and the proportion of abortive clones formed by WT keratinocytes or cells transduced with DeltaDS, which cannot bind syntenin (Fig. 4C, Table 4). However, knockdown of syntenin in keratinocytes expressing DeltaFl reduced the CFE and increased the proportion of abortive clones (Fig. 4C, Table 4).
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We next performed mixing experiments, comparing the behaviour of GFP labelled cells expressing DeltaFl alone, Rnai1 alone or in combination (Fig. 4D,E). Knockdown of syntenin in WT cells did not affect cell cohesion. However, knockdown of syntenin in DeltaFl-expressing keratinocytes reduced the proportion of cohesive clones to the same extent as DeltaVA (Fig. 4D,E; Fig. 3B). As independent confirmation of the role of Delta1 in promoting keratinocyte cohesion, we generated Delta1-null mouse keratinocytes from mice expressing K5Cre x Deltaflox/flox (Hozumi et al., 2004) and found that these cells also scattered when combined with WT keratinocytes in the mixing experiments (Fig. 4F,G). Taken together, our results suggest that syntenin contributes to the effects of Delta1 on Notch activation, keratinocyte differentiation and intercellular adhesion.
Delta1 ubiquitylation and internalisation are essential for Notch signalling (Glittenberg et al., 2006; Itoh et al., 2003). The cytoplasmic domain of Delta1 is ubiquitylated via the RING ubiquitin ligase Mind bomb, which promotes Delta1 internalisation (Itoh et al., 2003). Since syntenin is involved in membrane trafficking (Fernandez-Larrea et al., 1999) and has been identified in early endosomes (Fialka et al., 1999; Latysheva et al., 2006; Thery et al., 2001) we speculated that syntenin plays a role in maintaining Delta1 at the cell surface. In support of this, in keratinocytes transduced with DeltaFl there was colocalisation of DeltaFl with endogenous syntenin at cell-cell borders (Fig. 5A-C). When Rnai1 was introduced, syntenin was no longer detectable by immunofluorescence staining (Fig. 5B,E) and there was a major reduction in the level of DeltaFl at the cell surface (Fig. 5D). The reduction in surface levels of DeltaFl correlated with an increase in DeltaFl-positive cytoplasmic vesicles, which have previously been shown to colocalise with a marker of late endosomes and lysosomes (Fig. 5D) (Lowell et al., 2000).
Based on these observations we predicted that DeltaVA, because of its inability to bind syntenin, would be cleared more rapidly from the cell surface than DeltaFl. Although DeltaDS cannot bind syntenin it also lacks the Mind-bomb-binding site (Glittenberg et al., 2006; Itoh et al., 2003) and so would not be expected to exhibit rapid internalisation. To examine this, we transduced keratinocytes with DeltaFl, DeltaDS or DeltaVA and incubated them at 4°C with the antibody to the extracellular domain of zebrafish Delta. After washing the cells extensively at 4°C to remove unbound antibody, they were transferred to 37°C, fixed after 0, 30, 90 or 240 min and the location of the antibody was examined (Fig. 5G-P). In control keratinocytes infected with the empty retroviral vector, no binding or internalisation of antibody was detected (data not shown).
By 30 minutes, 90% of cells retained DeltaFl on the cell surface; this fell to 80% at 90 minutes and 30% at 240 minutes (Fig. 5G-I,P). DeltaDS remained at the cell surface for longer: at 240 minutes 
70% of cells still had DeltaDS on the plasma membrane (Fig. 5J-L,P). By contrast, DeltaVA was rapidly cleared from the cell surface and by 30 minutes, only 10% of cells retained surface expression of the protein (Fig. 5M-P). The vesicular localisation of DeltaVA (Fig. 5N) was reminiscent of Delta Fl localisation when syntenin was knocked down (Fig. 5D). We verified by western blotting that the overall expression levels of the different Delta constructs and endogenous syntenin were similar (Fig. 5Q). The progressive loss of Delta from the cell surface is consistent with receptor internalisation (Latysheva et al., 2006), although we cannot exclude the possibility that some Delta recycling occurred (Paterson et al., 2003; Xiao et al., 2005). These experiments suggest that syntenin binding to the PDZ domain of Delta1 promotes retention of the protein at the cell surface.
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Discussion |
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; Jensen et al., 1999; Jones et al., 1995; Legg et al., 2003; Lowell et al., 2000). The stem cell clusters express high levels of Delta1, which promotes keratinocyte cohesiveness via a mechanism that is dependent on its cytoplasmic domain (Lowell et al., 2000; Lowell and Watt, 2001). We now show that Delta1-mediated cohesion depends on the Delta1 PDZ-binding domain and that mutation of this domain not only abolishes keratinocyte cohesion but also stimulates terminal differentiation by enhancing Notch signalling (Blanpain et al., 2006; Rangarajan et al., 2001). These results are summarised schematically in Fig. 6.
We have shown that Delta1-mediated cohesion depends on the interaction of the PDZ domain with syntenin. In addition, syntenin regulates Notch signalling by reducing Delta1 internalisation, accounting for the enhanced signalling and differentiation-promoting activity of DeltaVA. Whereas keratinocytes lacking endogenous Delta1 show reduced intercellular adhesion, syntenin knockdown in WT cells did not have the same effect. This probably reflects both heterogeneity of the WT cells (a mixture of stem and transit amplifying cells) and the ability of Delta1 to interact with additional proteins (Table 3). Since Delta1 can bind additional PDZ proteins, including synectin, Discs large and the MAGI-membrane-associated scaffolding proteins (Table 3) (Mizuhara et al., 2005; Six et al., 2003; Wright et al., 2004), there is further potential for Delta1 to participate in multiple signalling complexes at the cell surface.
Syntenin could promote Delta1-mediated cell adhesion by enabling Delta1 to remain at the cell surface for longer and/or via the ability of syntenin to interact with additional proteins. Syntenin has two PDZ domains, which are proposed to bind different proteins in the same subcellular location, resulting in assemblies of syndecans and other partners, such as ephrin-B1 and Delta1, in supramolecular assemblies (Lin et al., 1999). In keratinocytes, Delta1 colocalises with cortical actin, and overexpression of mutant Delta lacking most of the cytoplasmic domain stimulates cell spreading and motility, suggesting that the effect of Delta on cohesiveness is not solely attributable to increased intercellular adhesion (Lowell and Watt, 2001). Syntenin could potentially link Delta1 and integrins (De Joussineau et al., 2003; Julich et al., 2005), through its ability to bind syndecans, which in turn modulate integrin-mediated adhesion (Oh and Couchman, 2004).
Our observations support the view that Delta1, and indeed other Notch ligands, have two distinct functions: to initiate Notch signalling in a neighbouring cell and to initiate a PDZ-dependent signalling mechanism in the Delta1-expressing cell (Ascano et al., 2003; Itoh et al., 2003). We have shown that in cultured human interfollicular epidermis, the PDZ-binding domain of Delta1 regulates stem cell clustering and also Notch signalling through its association with syntenin. Thus, high expression of Delta1 contributes to the epidermal stem cell niche both by determining the physical location of the cells and by sending a differentiation stimulus to neighbouring transit-amplifying cells (Fig. 6).
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Materials and Methods |
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) were subcloned into the retroviral vector pBabe puro as described previously (Lowell et al., 2000). The DeltaVA point mutation construct was generated from pBabe puro-DeltaD FL using the Quikchange kit from Stratagene and the following oligos: 5'-CA ACC GAG GCG TAA CGC CAC AAC-3' and 5'-GTT GTG GCG TTA CGC CTC GGT TGC-3'. The syntenin GST construct was a kind gift from P. Zimmermann (Zimmermann et al., 2002). Hes1 luciferase reporter was a kind gift from U. Lendhal (Blokzijl et al., 2003). The following oligonucleotides were used for syntenin RNAi experiments: Oligo1, 5'-GAT CCC CAC TAT ATG GTG GCT CCT GTT TCA AGA GAA CAG GAG CCA CCA TAT AGT TTT TTA-3' and Oligo 2, 5'-AGC TTA AAA AAC TAT ATG GCT CCT GTT CTC TTG AAA CAG GAG CCA CCA TAT AGT GGG-3'. The oligos were cloned in pRetrosuper-IRES-GFP according to the Manufacturer's instructions (Oligoengine).
Antibodies
The following antibodies were used: Delta D, Zdd2 (kind gifts of J. Lewis), 6 integrin (GoH3; Serotec MCA699), keratin10 (Covance), transglutaminase1 (BC1; kind gift of R. Rice, University of California, Davis, CA), loricrin (DH11; rabbit antiserum to NH2-HQTQQKQAPTWPCK-COOH peptide), Anti-laminin5 (Polykal; kind gift of P. Marinkovitch, Stanford University, CA), Human Delta1 (biotinylated; R&D Systems; BAF1818) and syntenin (SYSY; 133002).
Cell culture and transfection
Primary human keratinocytes were isolated from neonatal foreskins of three different individuals (strains kj, kq and kv) and cultured in the presence of a feeder layer of J2-3T3 cells in FAD medium (one part Ham's F12, three parts Dulbecco's modified Eagle's medium, 1.8x10–4 M adenine), supplemented with 10% fetal calf serum (FCS) and a cocktail of 0.5 µg/ml of hydrocortisone, 5 µg/ml insulin, 10–10 M cholera enterotoxin and 10 ng/ml epidermal growth factor (HICE cocktail) (Lowell et al., 2000).
A431 and 293T cells were maintained in culture using Dulbecco's modification of Eagle's medium, supplemented with 10% fetal calf serum. 293T cells were transfected using lipofectamine reagent according to the manufacturer's instructions (InVitrogen) and analysed 48 hours after transfection.
Retroviral infection
Retroviruses were prepared by a two-step packaging procedure involving transient transfection of ecotropic Phoenix cells and infection of amphotropic AM12 cells with viral supernatant from the Phoenix cultures (Janes et al., 2004). Infection of keratinocytes with pBabe puro-GFP virus was carried out by co-culture with AM12 retrovirus producer cells (Lowell et al., 2000). Retroviral infection of keratinocytes or J2-3T3 cells with all the Delta constructs was carried out using virus-containing supernatant (Janes et al., 2004). When keratinocytes were to be doubly infected with a Delta retrovirus and pBabe puro-GFP, they were grown for at least 7 days in puromycin after infection with Delta constructs before harvesting and replating on GFP retroviral producer cells.
Clonogenicity assays
Keratinocytes were seeded at clonal density (500 cells per 60-mm-diameter dish), cultured for 14 days, then fixed and stained with Rhodanile Blue (Lowell et al., 2000). All colonies (that is, groups of 
2 cells) were scored on each dish and CFE was calculated as the percentage of all plated cells that formed colonies (Lowell et al., 2000). Colonies were scored as abortive if they contained fewer than 40 cells, the majority of the cells being large and terminally differentiated (Lowell et al., 2000).
Mixing experiments
Keratinocytes were infected with pBabe puro-DeltaFl (K-DeltaFL), pBabe puro-DeltaLC (K-DeltaLC), pBabe puro-DeltaDS (K-DeltaDS), pBabe puro-DeltaVA (K-DeltaVA), or empty vector (K-WT) and subsequently with GFP. GFP-labelled cells were co-cultured with either unlabelled K-WT or K-Delta. 104 GFP-labelled cells were mixed with 105 unlabelled WT cells from the same strain and passage number and seeded on 35-mm-diameter tissue culture dishes (Falcon). The cultures were grown for 5 days, fixed with 4% paraformaldehyde and then the proportion of cohesive clones determined under a UV microscope, as described previously (Lowell and Watt, 2001). All experiments were carried out at least five times. Significance levels were calculated using the Student's t-test.
Reconstituted human epidermis
Reconstituted human epidermis was prepared as described previously (Janes et al., 2004). 1.5 cm2 pieces of de-epidermised dermis were seeded with 105 retrovirally infected primary human keratinocytes and grown at the air-liquid interface in keratinocyte medium. DED cultures were harvested after 16 days and frozen immediately on dry ice for Haematoxylin and Eosin staining or immunohistochemical analysis.
Yeast two-hybrid screen
Yeast two-hybrid protein interaction experiments and library screenings were performed using the Matchmaker GAL4 two-hybrid system 3 (BD Bioscience), according to the manufacturer's instructions. The AH109 yeast host strain was co-transformed with the bait plasmid, consisting of the intracellular domain of mouse Dll1 in pGBKT7, and a mouse E17 embryo Matchmaker cDNA library in the cloning vector pGAD1 (Groot et al., 2004).
GST pull downs
Fusion proteins were expressed in Escherichia coli BL21 cells and purified from cell extracts using Glutathione-Sepharose 4B (Amersham Biosciences). Confluent cultures of A431 cells or transfected 293T cells were extracted in ice-cold buffer (20 mM Tris-HCl pH 8, 150 mM NaCl, 1% NP40) containing 10 mM EDTA and protease inhibitors (Roche). Protein samples were incubated by rotation at 4°C for 40 minutes with 10 µg GST or GST-syntenin prebound to Glutathione-Sepharose 4B. Precipitates were washed in ice-cold lysis buffer (20 mM Tris-HCl pH 8, 150 mM NaCl, 1% NP40) and boiled in SDS-PAGE sample buffer before gel electrophoresis.
Immunoprecipitation
Confluent cultures of A431 cells were extracted using ice-cold buffer (20 mM Tris-HCl pH 8, 150 mM NaCl, 1% NP40) containing 10 mM EDTA and protease inhibitors (Roche). Proteins were immunoprecipitated with anti-syntenin antibody or an irrelevant antibody (anti-laminin5) and immunoblots were probed with anti-human Delta1 antibody.
Luciferase assay
J2-3T3 cells infected with the different Delta constructs were transiently transfected with a Hes1 reporter (Jarriault et al., 1995) and a Renilla luciferase control vector (Janes et al., 2004). 24 hours after transfection, cells were extracted and the activity of the reporter was measured using the Promega dual luciferase kit, according to the manufacturer's instructions.
Delta1 internalisation
Human primary keratinocytes infected with different Delta constructs were grown on glass coverslips. Cells were incubated with 10 µg/ml mAb Zdd2 in serum-free FAD medium for 1 hour at 4°C. Cells were rinsed three times with ice-cold serum free FAD (zero time point) and then transferred to pre-warmed complete growth medium at 37°C for different length of time (30 minutes, 90 minutes and 240 minutes). At each time-point, cells were fixed with 2% paraformaldehyde for 20 minutes at room temperature. Cells were subsequently permeabilised in 0.1% Triton X-100, rinsed in PBS and blocked for 1 hour with blocking buffer (10% fetal calf serum and 0.25% fish skin gelatin). After blocking, cells were incubated with Alexa Fluor 488-conjugated secondary antibody, rinsed in PBS and water and analysed using a Zeiss confocal microscope. The percentage of cells that retained Delta1 on the plasma membrane at each time point was determined. At least 100 cells were counted per experimental condition and three independent experiments were performed.
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Acknowledgments |
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