spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Effects of syntenin knockdown or Delta1 mutation on cell surface expression of Delta mutants. (A-F) Keratinocytes transduced with DeltaFl and pRetrosuper control vector (A-C) or syntenin siRNA (Rnai1; D-F) were double labelled with anti-DeltaD (green A,D) and anti-syntenin (red B,E) antibodies. (C,F) Merged images of middle and left panels. Bar, 20 µm. (G-O) Primary human keratinocytes transduced with DeltaFl, DeltaDS or DeltaVA were incubated with an anti-Delta antibody at 4°C (t=0), then transferred to fresh medium at 37°C for the times indicated. Bars, 50 µm. (P) Percentage of cells with Delta antibody at the plasma membrane. Data are means ± s.e.m. of three independent experiments. 100 cells of each type were counted per experiment. (Q) Western blot of keratinocytes transduced with ev, DeltaFl, DeltaDS and DeltaVA constructs, probed with antibodies to Zf-Delta, syntenin and actin.





Right arrow Return to article