spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Molecular lesions, TAC-1 and ZYG-9 distribution in three new tac-1 temperature-sensitive mutant alleles. (A) Schematic representation of TAC-1 (1-260) showing the position and nature of the mutations identified in the three new tac-1 alleles. Cyan box, TACC domain. (B-I) One-cell-stage embryos during mitosis in the wild-type (B,C) and tac-1 temperature-sensitive mutant alleles shifted at 25°C for >12 hours (D-G) or maintained at 15°C (H,I). B,D,F,H: ZYG-9 and {alpha}-tubulin staining; C,E,G,I,: TAC-1 and {alpha}-tubulin staining. Left panels show ZYG-9 or TAC-1 staining alone, right panels the merge of ZYG-9 or TAC-1 (red), {alpha}-tubulin (green) and DNA (blue). The histograms on the right of each staining series represent the quantification of ZYG-9 and TAC-1 levels in the cytoplasm (white bars) and the ratio between the levels of ZYG-9 or TAC-1 at centrosomes and in the cytoplasm (black bars) (Materials and Methods). Values are given as the mean ± s.e.m.; a.u., arbitrary units relative to the wild-type values, which have been set to 1 (dashed lines). Numbers of embryos analyzed for TAC-1 and ZYG-9 were: wild type, 17 and 13; tac-1(or369) plus tac-1(or402): 19 and 18; tac-1(or455), 10 and 10; tac-1(or455) [15]: 11 and 14. Anterior is left, posterior is right; bars, =10 µm.





Right arrow Return to article