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Figure 6


Fig. 6. ZYG-8, through its doublecortin domain, interacts with TAC-1, in a ZYG-9-free complex. (A,B) GST-pull-down experiments with (A) in vitro translated [35S]-labeled full-length ZYG-8 (arrowhead) or an unrelated [35S]-labeled control protein (denoted `ctrl', see Materials and Methods), or (B) [35S]-labeled ZYG-8 fragments. Shown are 10% of the input material and 100% of the material pulled-down by either GST-TAC-1 or GST. Ponceau staining indicates that comparable amounts of GST-TAC-1 and GST had been incubated with the radioactive products (data not shown). (C) Co-immunoprecipitation experiments of wild-type embryonic extracts. Extracts were immunoprecipitated with TAC-1, ZYG-8 or ZYG-9 antibodies (indicated above the lanes), and the immunoprecipitated material was analyzed by western blot using antibodies against ZYG-9, ZYG-8 or TAC-1 (indicated on the left).





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