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Figure 5


Fig. 5. Aurora-A coordinates centrosome- and chromatin-independent spindle assembly. Aurora-A-coated beads assemble bipolar spindles in Xenopus egg extracts in the absence of chromatin and centrosomes, in combination with the EXTAH complex. Aurora-A (Aur) phosphorylates Tpx2, which in turn leads to the autophosphorylation of Aurora-A and hence activation of its kinase activity (in orange). Activation of Aurora-A kinase recruits other proteins of the EXTAH complex, which are required for MT nucleation ({gamma}TuRC), MT stabilisation (XMAP215) and MT bundling (HURP). Eg5 may provide motor activity, which, in combination with the MT activities mentioned, could slide apart, cross-link and stabilise MTs emanating from adjacent Aurora-A-coated beads. Once a bipolar spindle is formed, members of the EXTAH complex may help maintain the stability of this structure.





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