spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. A screen for TBC domain proteins that alter ERGIC structure. (A-C) HeLa cells were transfected with the GFP-tagged wild-type and catalytically inactive point mutant TBC-domain proteins listed in the figure. After 24 hours they were fixed, and then stained with antibodies to ERGIC53. (A) The number of cells displaying a fragmented ERGIC was then counted (n>100 per condition), expressed as a percentage, and then plotted as a bar graph: grey bars, wild-type GAPs; open bars, inactive mutants. (B) Cells stained for the expressed wild-type (WT) TBC1D20 and RN-tre, and ERGIC53 are shown. (C) Cells stained for the expressed catalytically inactive mutant (RA) TBC1D20 R105A and RN-tre R150A and ERGIC53 are shown. Bars, 10 µm, in all panels.





Right arrow Return to article