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Figure 8


Fig. 8. Loss of recycling ERGIC markers from ERES in TBC1D20-expressing cells. HeLa cells expressing GFP-tagged p24 (green) were transfected with wild-type (WT) or catalytically inactive (RA) Myc-tagged TBC1D20 (blue). After 18 hours cells were fixed and then stained with antibodies to either (A) the ERGIC marker ERGIC53 or (B) the Sec31 subunit of the COPII vesicle coat (red), the Myc epitope (blue). GFP was visualized directly (green). The enlargements correspond to 10x10 µm regions of the merged images. Bar indicates 10 µm in all panels.





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