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Figure 1


Fig. 1. Distribution of VASP within human PTECs. (a) Cells stained for VASP (green), actin (red) or merged with images of nuclei stained with DAPI (blue). The arrowhead in the upper panel indicates the edge of a lamellipodium. (b,c) Human PTECs were transfected with WT GFP-VASP (b) or GFP-S239A VASP (c), then fixed 16 hours after transfection and imaged for GFP (green) and nuclei stained with DAPI (blue). (d) Western blot of cell extracts from human PTECs either untransfected control (C) or transfected with WT GFP-VASP (WT) or S239A GFP-VASP (S239A). Molecular mass markers (kDa) are to the left of the panel. Arrowheads indicate bands of endogenous VASP (~50 kDa) and GFP-VASP fusion proteins (~75 kDa). The usual doublet band of VASP is not seen on this gel as it was optimized to show both endogenous and transfected protein. (e) Human PTECs transfected with a GFP control vector alone. (f) Untransfected human PTECs visualised using phase-contrast microscopy (upper panel) or GFP fluorescence (lower panel). Bars, 10 µm.





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