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Figure 8


Fig. 8. Effects of NO on F-actin and focal adhesions in PTECs. (a) Untreated cells stained for VASP (green), actin (red) and nuclei (blue). Arrowheads indicate focal adhesions, arrows indicate actin stress fibres. (b) Changes in F-actin/VASP in cells following addition of NO donor and the effects on lamellipodia. After 30-minute treatment with NO donor, cells were fixed and stained for VASP (green), actin (red) and nuclei (blue). Cellular actin was not seen in cell processes. Occasionally, cells retained a cortical actin cytoskeleton (arrows). (c) Changes in F-actin/VASP in cells following addition of NO donor and the effects on focal adhesions. Cells treated and stained as described for panel b. This panel shows specifically cells with focal adhesions (arrowheads) or actin stress fibres (arrow) that show no effect following treatment with NO donor. Bars, 10 µm.





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