spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Depolarization induces Ca2+-dependent nuclear translocation of PYK2 in hippocampal neurons in culture. (A) Rat hippocampal neurons in culture treated with high [K+]o (High K+, 40 mM) or with a control solution for 3 minutes in the presence or absence of EGTA (5 mM, added 5 minutes before depolarization) and stained with an anti-PYK2 antibody were analyzed by fluorescence microscopy. Bars, 5 µm. (B) Quantification of the percentage of cells with nuclear PYK2. Values are means ± s.e.m. (six coverslips per group). Two-way ANOVA: interaction between depolarization and EGTA treatment F(1,20)=48.35, P<0.0001, depolarization effect F(3,20)=62.12, P<0.0001, EGTA treatment effect F(1,20)=77.28, P<0.0001. Newman-Keuls test: PYK2 control vs K+, ***P<0.001, PYK2 K+ vs (PYK2 + EGTA K+), °°°P<0.001. (C) Rat hippocampal neurons in culture treated with high [K+]o (High K+, 40 mM) or with a control solution for 3 minutes and stained with an anti-PYK2 antibody were analyzed by confocal microscopy. A single optical section is shown. Bars, 5 µm.





Right arrow Return to article