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Figure 4


Fig. 4. GFP-ninein dynamics showing speckle release and movements away from the centrosome. (A-C) Numerous GFP-ninein speckles in the immediate environs of the centrosome in a PtK2 cell (A) were highly motile (see supplementary material Movie 1), and some had a string-like appearance [(B); arrows], which was also observed for endogenous ninein in an immuno-labelled non-transfected cell [(C); arrows]. (D) Image sequence (inverted) indicating initial release of string-like GFP-ninein followed by release of a speckle (arrows) from the centrosome in an ARPE-19 cell (see supplementary material Movie 4). (E,F) Image frames (inverted) taken from a time-lapse recording of a MDCKII cell showing a GFP-ninein speckle being released from the centrosome (E) and a speckle moving away from the centrosome (F), with arrows indicating the progress of the speckles at different time points (see supplementary material Movies 2 and 3). Time is shown in seconds (s). Bars, 2 µm.





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