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Figure 6


Fig. 6. GFP-ninein speckles travel along microtubules. (A,A') Tracking of GFP-ninein speckles moving away from the centrosome over successive frames reveals several speckles following the same path. The speckles are indicated by a coloured arrow in (A), and their paths are shown by the corresponding coloured lines in (A'). (B) Immuno-gold labelling for ninein in the centrosomal region showing gold particles (highlighted in yellow) along the wall of a microtubule (arrowhead) anchored to one of the subdistal appendages of the mother centriole. Also note gold particles associated with the subdistal appendices (arrows). (C) Time-lapse frames of the centrosomal region of a PtK2 cell expressing GFP-ninein (red) and YFP–{alpha}-tubulin (green) (see supplementary material Movie 5). GFP-ninein speckles appear associated with the radiating microtubules (arrows). The frames show distinct changes in ninein speckle distribution over time. Time is shown in seconds (s). Bars, 1 µm (A,A'), 100 nm (B), 2 µm (C).





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