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Figure 9


Fig. 9. Ninein localisation in polarised MDCKII cells. (A) Single confocal optical section through the apical region of a polarised MDCKII cell, with ninein at the centrosome, as speckles in the cytoplasm, and at the cell periphery where it forms a distinct punctate pattern (arrow). (B) Apical view of a 3D reconstruction (based on confocal optical sections) of three polarised cells showing ninein at the centrosome and accumulated within a peripheral ring at the location of the anchoring sites. (C) Merged image, with ninein (green) and beta-catenin (red), showing regions of substantial colocalisation (yellow) at the cell periphery. (D-F) Regions of colocalisation analysed using Volocity (Improvision) software, with (F) indicating regions with strong colocalisation (yellow). (G) Lateral view of a 3D reconstruction from a confocal Z stack of a polarised cell showing the distinct apical localisation of ninein (green). The nucleus is labelled with DAPI (blue). (H) Rotated image showing an oblique view of a 3D reconstruction of a group of four cells, with ninein (green) at the centrosomes and concentrated within apical peripheral rings. The nucleus is labelled with DAPI (blue). Bars, 5 µm (A,D,E,F), 5 µm (B,C), 2 µm (G,H).





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