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Fig. 9. Cell densities of wild-type (CU427.4) and mutant (DYH7neo3) cultures observed over time. (A) Mutant standing tube cultures (broken line) displayed a reduced growth rate when compared with wild-type CU427.4 standing tube cultures (solid line). (B) This growth-defect phenotype is rescued when the shaken flask culturing method is employed. Each bar and data point represents the mean ± s.d. of three trials of 100-cell measurements.