spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. mDia1 regulates MyoD by an SRF-independent pathway. (A) Schematic of full-length mDia1 (FL) and five truncation derivatives. (B,C) mDia1{Delta}N3 activates SRF. C2C12 myoblasts were co-transfected with full-length mDia1 (FL), mDia1 mutants or a GFP control, the SRF reporter and a beta-gal plasmid. To minimize effects of serum on SRF activity, transfected cells were incubated in 0.5% serum for 24 hours before assay. mDia{Delta}N3 increased SRF activity >25 fold (mean ± s.e.m., n=4, P<0.0041). Western blotting with anti-GFP (panel B, inset) showed that all mutants were expressed at relatively equal levels. (C) Quantification of MyoD expression detected by immunofluorescence assay in cells overexpressing GFP (control), full-length mDia1 (FL) or mDia1 mutants ({Delta}N3, Hind3, F2, H+P, CC). Despite strongly activating SRF, mDia1{Delta}N3 suppresses MyoD expression maximally (mean ± s.e.m., n=7, P<0.0001). (D) Immunodetection of MyoD expression in C2C12 myoblasts transiently transfected with GFP-tagged mDia1 truncation mutants ({Delta}N3, H+P, CC).





Right arrow Return to article