spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 8


Fig. 8. Overexpression of an APC-independent form of beta-catenin leads to functional bypass of mDia{Delta}N3 inhibition. (A) Myoblasts were co-transfected with a control plasmid (GFP), {Delta}N3+GFP or {Delta}N3+ beta-catenin S37A and TCF activity determined (mean ± s.e.m., n=5, P<0.0046). (B) MyoD expression in cells transfected as in A. Note that {Delta}N3+ beta-catenin S37A transfected cells retain the elongated morphology typical of {Delta}N3 transfectants but are MyoD+. (C) Quantification of MyoD expression in myoblasts transfected with either {Delta}N3+ beta-catenin S37A or {Delta}N3HindIII+ beta-catenin S37A. The degradation-resistant beta-catenin S37A mutant partially reverses the inhibition of MyoD expression mediated by both mDia derivatives. (mean ± s.e.m., **P<0.0002, n=6; *P<0.046, n=3).





Right arrow Return to article