|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 5. Exo84 genetically interacts with apical and basolateral components. (A-F) Wild-type cuticle at the end of embryogenesis (A). In Exo84 at 25°C (B) and crumbs (C) small scraps of cuticle form, whereas dlg (D) and lgl (E) produce a continuous, malformed cuticle. Exo84 mutants at 20°C exhibit a weak defect in cuticle formation resulting in small ventral holes (F). (G-I) In strongly defective embryos, little cuticle is present (G). In moderately defective embryos, defects range from large ventral holes (H) to embryos with patches of cuticle. A weak classification indicates small ventral holes (I). (J) Cuticle defects in combinations of Exo84 with mutations in epithelial polarity genes. Exo84 embryos at 20°C that carry mutations in crumbs, sec5 or sec6 exhibit a stronger defect in cuticle integrity than Exo84 alone. By contrast, a decrease in the dosage of the basolateral determinants dlg or lgl partially restored cuticle formation in Exo84 mutants at 25°C. Overexpression of crumbs in Exo84 embryos allowed partial cuticle formation, whereas overexpression of lgl in Exo84 enhanced the cuticular defects. Defects in Exo84 mutants at 25°C were not suppressed by crumbs overexpression. For mutant combinations of Exo84 and crumbs, all Exo84 mutant embryos received half the maternal dosage of crumbs and one half were predicted to be homozygous for crumbs. For mutant combinations of Exo84 and sec5, sec6, dlg or lgl, all embryos received half the maternal dosage of sec5, sec6, lgl or dlg and one quarter were predicted to be homozygous for sec5, sec6, lgl or dlg. For experiments in which crumbs or lgl were overexpressed in an Exo84 background, one quarter of the embryos were predicted to receive both the Gal4 driver and the UAS transgene (see Materials and Methods). Percentages represent the fraction of embryos that did not hatch. Anterior, left; ventral, down. Bar, 100 µm.
|
