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Fig. 7. Aggregation of polarity proteins in Exo84 mutant embryos. (A) Schematic depicting the distribution of Crumbs (Crb), Bazooka (Baz), Armadillo (Arm), and Rab11 in cross section in wild-type and Exo84 mutant embryos. In Exo84 mutants, these proteins colocalize in large aggregates. (B,C) Stage 10 Exo84 mutant embryos stained for Crumbs (green), Armadillo (red) and Bazooka (blue). In severely disrupted cells in which Crumbs, Armadillo and Bazooka proteins are absent from the apical surface (panel B, lower right), ectopic aggregates of these proteins occur basolaterally (C, shown 2 µm below B). Note that cells adjacent to the affected region lack apical Crumbs but maintain junctional Armadillo (B). (D) Aggregation of DE-cadherin (green) and Bazooka (red) in Exo84 mutant embryos, shown 4 µm below the apical surface. (E) Colocalization of Crumbs (red) with recycling endosome aggregates (Rab11, green) in Exo84 embryos. (F-I) An Exo84 embryo imaged at three positions along the apical-basal axis. In regions where Bazooka (blue) and Armadillo (red) are absent from the apical surface (F), large accumulations of Bazooka and Armadillo colocalized with recycling endosomes in z-planes 2 µm (G) and 4 µm (H) below the apical plane in (F). (I) An enlarged view of the boxed area in G. (J-M) In the wild type (J,L) and shg;Exo84 (K,M), aggregation of Crumbs (red L,M) and Bazooka (blue) occurs despite strongly reduced levels of Armadillo (green) and E-cadherin (red J,K). Anterior, left; ventral, down. Bars, 10 µm.

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