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Figure 3


Fig. 3. Intracellular distribution of endogenous Mlph and EB1 in wild-type melan-ink melanocytes. Melan-ink cells were fixed and stained using antibodies that specifically recognise EB1 (A) and Mlph (B) as described in Materials and Methods. Panel C is the corresponding transmitted light image showing the distribution of melanosomes, panel D is an inverted and filtered copy of the transmitted light images showing the position of melanosomes and panel E is a merged image showing EB1 (green), Mlph (red) and melanosomes (blue). Panels A`-E` are higher magnification images of the indicated regions of A-E. Bar, 20 µm.





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