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Figure 4


Fig. 4. EB1 comet movements in wild-type and melan-ln melanosomes. Wild-type and melan-ln melanocytes were transiently transfected with plasmid encoding EB1-EGFP and the movements of EB1 comets and melanosomes were recorded as described in Materials and Methods. Panel A shows the distribution of EB1-EGFP in a melan-ln melanocyte and a magnified section of this cell in which arrows indicate individual comet structures. Panel B shows the tracks of melanosomes (red) and EB1-EGFP comets (green) in individual transfected wild-type and melan-ln cells over the course of a 95 second time-lapse recording (these tracks correspond to Movies 1 and 2 in supplementary material). Bars, 10 µm (A); 5 µm (B). Panel C shows the velocity of EB1-EGFP comets in representive wild-type ({blacksquare}) and melan-ln ({square}) melanocytes. The velocities of individual comets (n=2541 comets from 20 cells for wild-type and n=1711 comets from 10 cells for melan-ln) were binned into velocity ranges of 0.025 µm/sec and then plotted showing the percentage of the total population that falls within each bin.





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